Breath condenser coatings affect measurement of biomarkers in exhaled breath condensate.
نویسندگان
چکیده
Exhaled breath condensate collection is not yet standardised and biomarker measurements are often close to lower detection limits. In the current study, it was hypothesised that adhesive properties of different condenser coatings interfere with measurements of eicosanoids and proteins in breath condensate. In vitro, condensate was derived from a collection model using two test solutions (8-isoprostane and albumin) and five condenser coatings (silicone, glass, aluminium, polypropylene and Teflon). In vivo, condensate was collected using these five coatings and the EcoScreen condenser to measure 8-isoprostane, and three coatings (silicone, glass, EcoScreen) to measure albumin. In vitro, silicone and glass coatings had significantly higher albumin recovery compared with the other coatings. A similar trend was observed for 8-isoprostane recovery. In vivo, median (interquartile range) 8-isoprostane concentrations were significantly higher using silicone (9.2 (18.8) pg.mL(-1)) or glass (3.0 (4.5) pg.mL(-1)) coating, compared with aluminium (0.5 (2.4) pg.mL(-1)), polypropylene (0.5 (0.5) pg.mL(-1)), Teflon (0.5 (0.0) pg.mL(-1)), and EcoScreen (0.5 (2.0) pg.mL(-1)). Albumin in vivo was mainly detectable using glass coating. In conclusion, a condenser with silicone or glass coating is more efficient for measurement of 8-isoprostane or albumin in exhaled breath condensate, than EcoScreen, aluminium, polypropylene or Teflon. Guidelines for exhaled breath condensate standardisation should include the most valid condenser coating to measure a specific biomarker.
منابع مشابه
Exhaled breath condensate is not suitable to detect EGFR somatic mutations.
REFERENCES 1 Rosias PP, Jobsis Q, van de Kant K, et al. Global condensation: a ‘‘climate change’’ towards better standardisation of exhaled breath condensate measurements. Eur Respir J 2008; 31: 684–685. 2 Liu J, Conrad DH, Chow S, Tran VH, Yates DH, Thomas PS. Collection devices influence the constituents of exhaled breath condensate. Eur Respir J 2007; 30: 807–808. 3 Prieto L, Ferrer A, Palop...
متن کاملساخت نمونه بردار کندانس هوای بازدمی جهت مطالعه بیومارکرهای بازدمی استرس اکسیداتیو
Background: The use of exhaled breath condensate (EBC) biomarkers to evaluate exposure and disease in recent years gained attention in toxicology and occupational medicine. However, use of standard sampling apparatus is missing element in this procedure. Methods: Initial design of sampler was selected based on the water condensation system. The effect of sampler length, sampling time and tempe...
متن کاملExcessive daytime sleepiness in patients with sleep-disordered breathing.
REFERENCES 1 Liu J, Conrad DH, Chow S, Tran VH, Yates DH, Thomas PS. Collection devices influence the constituents of exhaled breath condensate. Eur Respir J 2007; 30: 807–808. 2 Rosias PP, Robroeks CM, Niemarkt HJ, et al. Breath condenser coatings affect the measurement of biomarkers in exhaled breath condensate. Eur Respir J 2006; 28: 1036–1041. 3 Horváth I, Hunt J, Barnes PJ. Exhaled breath ...
متن کاملIsolated diastolic dysfunction of right ventricle: stress-induced pulmonary hypertension.
REFERENCES 1 Horváth I, Hunt J, Barnes PJ. Exhaled breath condensate: methodological recommendations and unresolved questions. Eur Respir J 2005; 26: 523–548. 2 Hunt JF, Fang K, Malik R, et al. Endogenous airway acidification: implications for asthma pathophysiology. Am J Respir Crit Care Med 2000; 161: 694–699. 3 Kullmann T, Barta I, Lázár Z, et al. Exhaled breath condensate pH standardised fo...
متن کاملBiomarker reproducibility in exhaled breath condensate collected with different condensers.
Optimal collection and analysis of exhaled breath condensate (EBC) are prerequisites for standardisation and reproducibility of assessments. The present study aimed to assess reproducibility of EBC volume, hydrogen peroxide (H(2)O(2)), 8-isoprostane and cytokine measurements using different condensers, including a newly developed glass condenser. At four points in time, 30 healthy subjects perf...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- The European respiratory journal
دوره 28 5 شماره
صفحات -
تاریخ انتشار 2006